Substrate‐Independent Coating with Persistent and Stable Antifouling and Antibacterial Activities to Reduce Bacterial Infection for Various Implants

Abstract Implantation of biomedical devices accompanying infections has caused severe problems to public health that require feasible solutions. In this study, a simple approach is reported to fabricate a antimicrobial and antifouling dual‐functional coating. This coating consists of a substrate‐independent layer‐by‐layer (LBL) film formed by poly (diallyldimethylammonium) (PDDA) and poly (styrenesulfonate) (PSS), where parts of PSS and PDDA are physically substituted by hetero‐bifunctional polyethylene glycol (PEG) ending with a carboxyl group and antimicrobial peptide (ε‐Poly‐l‐lysine, ε‐PL). This design (ε‐PL‐PEG‐(PDDA/PSS)9 coating) exhibits not only potent antimicrobial activity against Gram‐positive/negative bacteria but also superior antifouling activity on various substrates, including glass and plastic. Moreover, the antifouling and antibacterial performance can be maintained for a longer period of time under physiological environments even after physical damage of the surface due to the homogeneous interspersion and free migration of ε‐PL‐PEG‐COOH in the LBL film. This allows the supplement of these molecules to the surface against molecule loss during usage. Both in vitro and in vivo (rodent subcutaneous infection model) studies show obvious reduction of the bacteria on the coated substrate and in the surrounding tissues with up to 3.2‐log reduction, even after repeated usage. The inflammation around the implantation area is also significantly inhibited.