材料科学
干细胞
纳米探针
近红外光谱
生物相容性
纳米材料
纳米颗粒
生物医学中的光声成像
纳米技术
生物医学工程
光学
医学
生物
遗传学
物理
冶金
作者
Chao Yin,Guohua Wen,Chao Liu,Boguang Yang,Sien Lin,Jiawei Huang,Pengchao Zhao,Siu Kei Wong,Kunyu Zhang,Xiaoyu Chen,G. Li,Xiaohua Jiang,J.C. Huang,Kanyi Pu,Lihong V. Wang,Liming Bian
出处
期刊:ACS Nano
[American Chemical Society]
日期:2018-11-15
卷期号:12 (12): 12201-12211
被引量:107
标识
DOI:10.1021/acsnano.8b05906
摘要
Photoacoustic (PA) imaging and tracking of stem cells plays an important role in the real-time assessment of cell-based therapies. Nevertheless, the limitations of conventional inorganic PA contrast agents and the narrow range of the excitation wavelength in the first near-infrared (NIR-I) window hamper the applications of PA imaging in living subjects. Herein, we report the design and synthesis of a second near-infrared (NIR-II) absorptive organic semiconducting polymer (OSP)-based nanoprobe (OSPN+) for PA imaging and tracking of stem cells. Comparison studies in biological tissue show that NIR-II light excited PA imaging of the OSPN+ has significantly higher signal-to-noise ratio than NIR-I light excited PA imaging, thereby demonstrating the superiority of the OSPN+ for deep tissue imaging. With good biocompatibility, appropriate size, and optimized surface property, the OSPN+ shows enhanced cellular uptake for highly efficient PA labeling of stem cells. In vivo investigations reveal significant NIR-II PA contrast enhancement of the transplanted OSPN+-labeled human mesenchymal stem cells by 40.6- and 21.7-fold in subcutaneous and brain imaging, respectively, compared with unlabeled cases. Our work demonstrates a class of OSP-based nanomaterials for NIR-II PA stem cell imaging to facilitate a better understanding and evaluation of stem cell-based therapies.
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