聚对苯二甲酸乙二醇酯
酵母
毕赤酵母
化学
环境友好型
降级(电信)
生物催化
酶
生物化学
材料科学
水解
重组DNA
生物
计算机科学
催化作用
复合材料
离子液体
基因
电信
生态学
作者
Zhuozhi Chen,Yunjie Xiao,Gert Weber,Ren Wei,Zefang Wang
出处
期刊:Methods in Enzymology
日期:2021-01-01
卷期号:: 457-477
被引量:9
标识
DOI:10.1016/bs.mie.2020.12.030
摘要
Enzymatic hydrolysis of polyethylene terephthalate (PET) is considered to be an environmentally friendly method for the recycling of plastic waste. Recently, a bacterial enzyme named IsPETase was found in Ideonella sakaiensis with the ability to degrade amorphous PET at ambient temperature suggesting its possible use in recycling of PET. However, applying the purified IsPETase in large-scale PET recycling has limitations, i.e., a complicated production process, high cost of single-use, and instability of the enzyme. Yeast cell surface display has proven to be an effectual alternative for improving enzyme degradation efficiency and realizing industrial applications. This chapter deals with the construction and application of a whole-cell biocatalyst by displaying IsPETase on the surface of yeast (Pichia pastoris) cells.
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