全氟辛酸
全氟辛烷
生物传感器
生物搬运器
磺酸盐
环境化学
自来水
化学
检出限
荧光
分子信标
废水
报告基因
色谱法
环境科学
生物化学
有机化学
基因表达
基因
环境工程
寡核苷酸
物理
钠
量子力学
作者
Sunantha Ganesan,Namasivayam Vasudevan
标识
DOI:10.1016/j.scitotenv.2020.143544
摘要
Abstract A simple, reagent and pre-treatment (i.e. dilution, sample purification and pH adjustment) free approach based genetically engineered bacterial biosensor is developed and demonstrated for the detection of perfluorinated compounds in water samples. The bacterial biosensor was developed by integrating two genes called regulatory (defluorinase gene) and reporter gene (green fluorescence gene) through genetic engineering techniques. The as-developed bacterial biosensor was employed to detect perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) in water samples upon induction of regulatory gene and expression of green fluorescence protein. The induced fluorescence emission by the biosensor was visualized using fluorescence microscopic images. The specificity of biosensor was evaluated with different types of organic pollutants such as chlorinated compounds, polyaromatic hydrocarbons and pesticides etc., in both presence and absence of PFOA and PFOS. The biosensor was employed to detect the perfluorinated compounds at nano gram level in both standard solutions and natural water samples like river water, wastewater and drinking water with an analysis time of 24 h. The detection of PFOA and PFOS by the developed-bacterial sensor is validated by liquid chromatography coupled with mass spectrometer. The developed biosensor has demonstrated a rapid and sensitive detection of PFOA and PFOS in various water samples.
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