A method for detecting perfluorooctanoic acid and perfluorooctane sulfonate in water samples using genetically engineered bacterial biosensor

Abstract A simple, reagent and pre-treatment (i.e. dilution, sample purification and pH adjustment) free approach based genetically engineered bacterial biosensor is developed and demonstrated for the detection of perfluorinated compounds in water samples. The bacterial biosensor was developed by integrating two genes called regulatory (defluorinase gene) and reporter gene (green fluorescence gene) through genetic engineering techniques. The as-developed bacterial biosensor was employed to detect perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) in water samples upon induction of regulatory gene and expression of green fluorescence protein. The induced fluorescence emission by the biosensor was visualized using fluorescence microscopic images. The specificity of biosensor was evaluated with different types of organic pollutants such as chlorinated compounds, polyaromatic hydrocarbons and pesticides etc., in both presence and absence of PFOA and PFOS. The biosensor was employed to detect the perfluorinated compounds at nano gram level in both standard solutions and natural water samples like river water, wastewater and drinking water with an analysis time of 24 h. The detection of PFOA and PFOS by the developed-bacterial sensor is validated by liquid chromatography coupled with mass spectrometer. The developed biosensor has demonstrated a rapid and sensitive detection of PFOA and PFOS in various water samples.