Carbon quantum dots as fluorescence sensors for label-free detection of folic acid in biological samples

Carbon quantum dots (CQDs) have been fabricated by a facile single-step pyrolysis method from citric acid and ethylene imine polymer. When excited at 359 nm, CQDs show intense blue fluorescence at 434 nm. The fluorescence can be effectively quenched by folic acid (FA), which is attributed to the combination of static quenching and inner filter effect. Thus, the CQDs are developed as an efficient fluorescent sensing platform for label-free sensitive and selective detection of FA. Key parameters influencing the detection were investigated, such as incubation time, salt concentration, selectivity and potential interferences. Under the optimal conditions, a good linearity was observed for the emission intensity against 1.14-47.57 μM with a correlation coefficient of 0.99. The limit of detection was found to be 0.38 μM. The practical application of the sensing system was demonstrated by analyzing FA in human urine samples. The sample recoveries fell in the range of 82.0%-113.1% with RSDs ≤ 10.9%, which presented its reliable and feasible application in real samples.