菌盖
菌柄(真菌学)
灵芝
抗氧化剂
化学
蘑菇
伞菌纲
生物化学
灵芝
食品科学
生物
植物
擔子菌門
作者
Xujiao Ren,Juying Wu,Lingling Huang,Kaisen Cheng,Meifang Zhang,Hailong Yang
出处
期刊:International Journal of Medicinal Mushrooms
日期:2020-01-01
卷期号:22 (2): 133-144
被引量:8
标识
DOI:10.1615/intjmedmushrooms.2020033683
摘要
Total phenolics, flavonoids, and polysaccharides, and individual ganoderic acid (GA) contents, antioxidant capacity, and transcription levels of key enzyme genes involved in GA biosynthesis in pileus and stipes of Ganoderma lucidum fruiting body at different growth stages were investigated in this study. Results showed that the highest total phenolics and total flavonoids contents were determined in stipes at spore maturity stage, resulting in high antioxidant activity, while the highest total polysaccharide content was found in pileus at the same stage. The pileus contained more GA than the stipes, and higher contents of ganoderic acid A and D were found at fruiting body mature stage while that of ganoderic acid B, C2, and G were found at bud elongation stage. Results from quantitative real-time PCR indicated that higher gene transcription levels of hydroxyl methylglutaryl-CoA reductase (hmgr), farnesyl pyrophosphate synthase (fps), squalene synthase (sqs), and oxidosqualene cyclase (osc) were found in pileus at bud elongation stage. Our findings will be helpful for understanding the biosynthesis of bioactive components and determining the harvest time for the desired G. lucidum fruiting bodies.
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