小檗碱
粪肠球菌
生物膜
抗菌活性
最小抑制浓度
微生物学
细胞毒性
MTT法
细菌
化学
最低杀菌浓度
体外
金黄色葡萄球菌
生物
抗菌剂
生物化学
遗传学
作者
Tao Gong,Yujia Cui,Xingwang Zhou,Boyu Tang,Baiping Ren,Yuqing Li
出处
期刊:PubMed
日期:2020-11-01
卷期号:51 (6): 749-754
被引量:1
摘要
To study the antibacterial effect of berberine combined with amylmetacresol on Enterococcus faecalis.Both dilution method and live bacteria CFU were used to determine the minimum inhibitory concentration (MIC) of berberine and amylmetacresol on E. faecalis. The killing effect of berberine and amylmetacresol on planktonic E. faecalis was detected by suspension quantitative germicidal test and live/dead bacteria staining. The effects of berberine and amylmetacresol on the structure of mature biofilm of E. faecalis was observed by scanning electron microscopy (SEM). The toxicity of berberine and amylmetacresol on human oral keratinocytes (HOK) was determined by CCK-8 cell proliferation and cytotoxicity assay and cytotoxicity LDH assay.The MIC of berberine was 512 μg/mL, and the MIC of amylmetacresol was 0.023 3%. 512 μg/mL berberine and 0.002 33% amylmetacresol had a weak killing effect on planktonic E. faecalis alone, while they showed a synergistic antibacterial effect in combination. Cell survival in the biofilm was only slightly changed by berberine and amylmetacresol. The structure of biofilm was obviously changed by berberine and amylmetacresol. 512 μg/mL berberine and 0.002 33% amylmetacresol alone or in combination showed the survival rate was much higher than the injury rate, suggesting berberine and amylmetacresol had a low cytotoxicity.Berberine and amylmetacresol had synergism against E. faecalis, and the biological safety of the combination use was better.
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