狼疮性肾炎
入射(几何)
医学
免疫学
肾炎
内科学
疾病
数学
几何学
作者
Dongjiang Liao,Xiping Cheng,Nan Li,Kang-Li Liang,Hui Fan,Suiying Zhang,Xiaoqian Hu,Ping Fan,Yuansheng Wu
出处
期刊:Combinatorial Chemistry & High Throughput Screening
[Bentham Science]
日期:2020-04-16
卷期号:23 (7): 649-657
被引量:3
标识
DOI:10.2174/1386207323666200416151836
摘要
Lupus nephritis (LN) is one of the major complications of systemic lupus erythematosus (SLE). The specific mechanisms of pathogenesis, aggravation, and remission processes in LN have not been clarified but is of great need in the clinic. Using isobaric tags for relative and absolute quantitation (iTRAQ) technology to screen the functional proteins of LN in mice. Especially under intervention factors of lipopolysaccharide (LPS) and dexamethasone.Mrl-lps mice were intervened with LPS, dexamethasone, and normal saline (NS) using intraperitoneal injection, and c57 mice intervened with NS as control. The anti-ANA antibody enzyme-linked immunosorbent assay (ELISA) was used to verify disease severity. Kidney tissue is collected and processed for iTRAQ to screen out functional proteins closely related to the onset and development of LN. Western blot method and rt-PCR (real-time Polymerase Chain Reaction) were used for verification.We identified 136 proteins that marked quantitative information. Among them, Hp, Igkv8-27, Itgb2, Got2, and Pcx proteins showed significant abnormal manifestations.Using iTRAQ methods, the functional proteins Hp, Igkv8-27, Itgb2, Got2, and Pcx were screened out for a close relationship with the pathogenesis and development of LN, which is worth further study.
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