Gene Expression Alterations Associated with Sanguinarine‐Induced Antiproliferative effects and Apoptosis in Triple‐Negative Breast Cancer Cells

The FASEB JournalVolume 35, Issue S1 PathologyFree Access Gene Expression Alterations Associated with Sanguinarine-Induced Antiproliferative effects and Apoptosis in Triple-Negative Breast Cancer Cells Samia Messeha, Samia Messeha Pharmaceutical Sciences, Florida A&M University, Tallahassee, FLSearch for more papers by this authorNajla Zarmouh, Najla Zarmouh Pharmaceutical Sciences, Florida A&M University, Tallahassee, FLSearch for more papers by this authorSophie Noel, Sophie Noel Pharmaceutical Sciences, Florida A&M University, Tallahassee, FLSearch for more papers by this authorKaram Soliman, Karam Soliman Pharmaceutical Sciences, Florida A&M University, Tallahassee, FLSearch for more papers by this author Samia Messeha, Samia Messeha Pharmaceutical Sciences, Florida A&M University, Tallahassee, FLSearch for more papers by this authorNajla Zarmouh, Najla Zarmouh Pharmaceutical Sciences, Florida A&M University, Tallahassee, FLSearch for more papers by this authorSophie Noel, Sophie Noel Pharmaceutical Sciences, Florida A&M University, Tallahassee, FLSearch for more papers by this authorKaram Soliman, Karam Soliman Pharmaceutical Sciences, Florida A&M University, Tallahassee, FLSearch for more papers by this author First published: 14 May 2021 https://doi.org/10.1096/fasebj.2021.35.S1.00463 This research was supported by NIH Grants U54 MD007582 and P20 MD 006738. About ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Sanguinarine (SAN) is a plant alkaloid found in various plants, mainly Sanguinaria canadensis. SAN has various pharmacological properties, including anti-bacterial, anti-fungal, anti-inflammatory, and anti-cancer effects. The current study investigated the mechanisms that mediated the anti-cancer effects of SAN in two triple-negative breast cancer (TNBC) cell lines; MDA-MB-231 and MDA-MB-468 cells. The data obtained suggested SAN is a potent compound in reducing cell viability and proliferation. Moreover, the results show that MDA-MB-468 cells were more sensitive than MDA-MB-231 cells to SAN. Also, SAN induced apoptosis in both cell lines by arresting cell growth. The compound arrested MDA-MB-231 cells at the Sub-G1 phase and MDA-MB-468 cells at the G2/M phase. Gene expression profiling indicated that MDA-MB-468 cells had significant transcription activation (2-4-fold) in eighteen genes. The genes activated include two members of the caspase family (caspase 1 and caspase 10), seven members of the TNF receptor superfamily (TNFRSF) such as TNFRSF10A, TNFRSF10B, TNFRSF11B, TNFRSF21, and TNFRSF25), in addition to TNFRSF1A Associated Via Death Domain (TRADD) and Fas-associated via death domain (FADD). On the other hand, in MDA-MB-231 cells, SAN was found to alter twelve five genes. The upregulated genes include Lymphotoxin Alpha (LTA; 15-fold), growth arrest and DNA damage-inducible 45 alpha (GADD45A), BCL2 Related Protein A1 (BCL2A1), BCL2-Like 11 (BCL2L11), and Bcl-2-Binding Protein Bis (BAG3). These results provide evidence that genetically different cells may respond differently to treatments, explaining the inadequate therapeutic response of some patients with advanced TNBC. The data also suggest that this plant alkaloid SAN could have therapeutic potential for TNBC patients. Volume35, IssueS1Special Issue: Experimental Biology 2021 Meeting AbstractsMay 2021 RelatedInformation