诱导多能干细胞
重编程
SOX2
KLF4公司
祖细胞
干细胞
脐带
细胞生物学
内皮干细胞
生物
再生医学
外周血单个核细胞
免疫学
脐带血
体细胞
胚胎干细胞
细胞
遗传学
体外
基因
作者
Xiugong Gao,Jeffrey J. Yourick,Robert L. Sprando
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 381-396
被引量:8
标识
DOI:10.1007/7651_2021_372
摘要
Induced pluripotent stem cells (iPSCs) offer the potential to generate tissue cells with donor diversity therefore promising to have widespread applications in regenerative medicine, disease modeling, drug discovery, and toxicity testing. Several somatic cell types have been utilized, with varying efficiencies, as source cells for the reprogramming of iPSCs. Recently, it has been reported that endothelial progenitor cells (EPCs) derived from umbilical cord blood (CB) or adult peripheral blood (PB) afford a practical and efficient cellular substrate for iPSC generation, and possess several advantages over other cell types. In this chapter, we describe a protocol that covers all steps of reprogramming iPSCs from blood-derived EPCs, including (1) isolation of mononuclear cells (MNCs) from blood samples, (2) derivation of EPCs from MNCs, and (3) generation of iPSCs from EPCs. The final step of reprogramming EPCs into iPSCs is achieved through ectopic expression of four transcription factors, OCT4, KLF4, SOX2, and c-MYC, using self-replicative RNA (srRNA) technology.
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