材料科学
生物相容性
组织工程
膜
接触角
静电纺丝
纤维
生物医学工程
纳米孔
复合材料
神经组织工程
表面改性
生物材料
化学工程
聚合物
纳米技术
化学
医学
生物化学
工程类
冶金
作者
Tao Xu,Xu Zhang,Xiu Dai
标识
DOI:10.1002/mame.202200256
摘要
Abstract It is important for the functional recovery of defective nerves to construct a suitable microenvironment for nerve regeneration by optimizing the topological structure and biological functions of nerve grafts. Electrospun fibers are employed to mimic the extracellular matrix (ECM) and the effect of the porous features on fiber surface draws much attention. Yet, little is known about the effect of the nanotopology of the fiber surface in conjunction with collagen I (COL) on neural cells. Herein, poly(lactic acid) (PLA)/COL hybrid fibrous membranes with a nanoporous structure on a fiber surface are fabricated. The performance of the obtained fibrous membranes is investigated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), water contact angle (WCA), and tensile test measurements. The cytotoxicity and biocompatibility of PLA/COL hybrid fibrous membranes and their effects on neural cells are systematically investigated. The physicochemical test results show that the PLA/COL hybrid fibrous membranes have appropriate mechanical properties and improved hydrophilicity. The MTT assay and hemolysis assay suggest the nontoxicity and good hemocompatibility of PLA/COL membranes. The nanoporous structure on the fiber surface and the introduction of COL synergistically promote proliferation and elongation of Schwann cells (SCs) and the extension of dorsal root ganglion (DRG) neurites.
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