肿瘤微环境
免疫系统
癌症研究
肝细胞癌
生物
免疫检查点
单细胞测序
转移
循环肿瘤细胞
癌变
癌症干细胞
癌症
免疫疗法
医学
免疫学
基因
突变
生物化学
遗传学
外显子组测序
作者
Xinyue Li,Yue Shen,Li Zhang,Xiao Guo,Jian Wu
标识
DOI:10.1016/j.bbcan.2022.188720
摘要
Unsatisfied clinical outcome drives to better understand hepatic carcinogenesis, microenvironment and escape of immune surveillance in hepatocellular carcinoma (HCC). Single cell RNA sequencing (scRNA-Seq) has generated enormous data to pinpoint pathophysiologic alterations in tumor microenvironment (TME) or trace lineage development in cancer stem cells (CSCs), circulating tumor cells (CTCs), and subsets of immune cells, such as exhausting T cells, tumor-associated macrophages (TAMs), dendritic cells or other lineages. New insights have significantly advanced current understanding in progression, poor responses to molecular-targeted therapeutics or immune checkpoint inhibitors, metastasis in both basic research and clinical practice. The present review intends to cover a basic workflow of the scRNA-seq technology, existing limitations and improvement areas. Moreover, in-depth understanding in TME, exhausting T cells, CSCs, CTCs, tumor-associated macrophages, dendritic cells in HCC facilitates implementation of personalized and precise therapy in an era of availability with an array of systemic regimens.
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