Testing of candidate probiotics to prevent dental caries induced by Streptococcus mutans in a mouse model

变形链球菌 微生物学 益生菌 殖民地化 生物 生物膜 血链球菌 唾液 细菌 遗传学 生物化学
作者
David J. Culp,William E. Hull,Alexander C. Schultz,Ashley S. Bryant,Claudia A. Lizarraga,Madeline R. Dupuis,Brinta Chakraborty,Kyulim Lee,Robert A. Burne
出处
期刊:Journal of Applied Microbiology [Wiley]
卷期号:132 (5): 3853-3869 被引量:4
标识
DOI:10.1111/jam.15516
摘要

We evaluated two species of human oral commensal streptococci in protection against dental caries induced by Streptococcus mutans.Candidate probiotics, Streptococcus sp. A12, Streptococcus sanguinis BCC23 and an arginine deiminase mutant of BCC23 (∆arcADS) were tested for their ability to reduce S. mutans-induced caries in an established mouse model. Mice were colonized with a probiotic, challenged with S. mutans, then intermittently reinoculated with a probiotic strain. Oral colonization of each strain and autochthonous bacteria was assessed by quantitative polymerase chain reaction. Both BCC23 strains, but not A12, were associated with markedly reduced sulcal caries, persistently colonized mucosal and dental biofilms, and significantly lowered S. mutans counts. All three strains enhanced mucosal colonization of autochthonous bacteria. In a follow-up experiment, when S. mutans was established first, dental and mucosal colonization of S. mutans was unaltered by a subsequent challenge with either BCC23 strain. Results between BCC23 and BCC23 ∆arcADS were equivalent.BCC23 is a potential probiotic to treat patients at high caries risk. Its effectiveness is independent of ADS activity, but initial dental cleaning to enhance establishment in dental biofilms may be required.In vivo testing of candidate probiotics is highly informative, as effectiveness is not always reflected by genotype or in vitro behaviours.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
xn201120完成签到 ,获得积分10
1秒前
大福发布了新的文献求助10
2秒前
xn201120关注了科研通微信公众号
4秒前
彭a完成签到,获得积分10
5秒前
藏识完成签到,获得积分10
8秒前
以甲引丁发布了新的文献求助10
8秒前
优秀剑愁完成签到 ,获得积分10
13秒前
小张z完成签到,获得积分10
13秒前
大福完成签到,获得积分10
14秒前
机灵晓刚完成签到 ,获得积分10
17秒前
羊毛毛衣完成签到,获得积分10
18秒前
橘涂完成签到 ,获得积分10
20秒前
23秒前
千里共婵娟完成签到,获得积分10
25秒前
刻苦冷菱完成签到 ,获得积分10
28秒前
XZY发布了新的文献求助10
29秒前
31秒前
酷酷的思萱完成签到,获得积分10
32秒前
早日发nature完成签到 ,获得积分10
33秒前
雨齐完成签到,获得积分10
33秒前
善学以致用应助ZSmile采纳,获得20
36秒前
tong发布了新的文献求助10
36秒前
朴实的小萱完成签到 ,获得积分10
37秒前
酷波er应助1huiqina采纳,获得30
37秒前
嗨嗨完成签到,获得积分10
38秒前
41秒前
41秒前
醉生梦死完成签到 ,获得积分10
42秒前
Axel完成签到,获得积分10
43秒前
秀丽白凝发布了新的文献求助20
43秒前
JamesPei应助奇奇吃面采纳,获得10
44秒前
天涯倦客完成签到,获得积分10
44秒前
万能图书馆应助叶孤城采纳,获得10
45秒前
Kk发布了新的文献求助10
46秒前
wlp鹏完成签到,获得积分10
46秒前
踏雪无痕完成签到,获得积分10
51秒前
52秒前
52秒前
53秒前
53秒前
高分求助中
Sustainability in Tides Chemistry 2800
Kinetics of the Esterification Between 2-[(4-hydroxybutoxy)carbonyl] Benzoic Acid with 1,4-Butanediol: Tetrabutyl Orthotitanate as Catalyst 1000
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Handbook of Qualitative Cross-Cultural Research Methods 600
Very-high-order BVD Schemes Using β-variable THINC Method 568
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3137627
求助须知:如何正确求助?哪些是违规求助? 2788531
关于积分的说明 7787471
捐赠科研通 2444861
什么是DOI,文献DOI怎么找? 1300119
科研通“疑难数据库(出版商)”最低求助积分说明 625814
版权声明 601023