分子印迹聚合物
化学
环糊精
转铁蛋白
组合化学
检出限
色谱法
选择性
生物化学
催化作用
作者
Pan Zhang,Gang Chen,Zhun Wang,Jiutong Ma,Qiong Jia
标识
DOI:10.1016/j.snb.2022.131669
摘要
As an important clinical biomarker, transferrin (TRF) plays an indispensable role in the function regulation of human body and its concentration reflects the occurrence of some diseases. Herein, a sensor based on magnetic molecularly imprinted polymers (MMIPs) and surface-enhanced Raman scattering (SERS) was developed for the sensitive detection of TRF. For this purpose, Fe3O4@Au@cyclodextrin-MIPs were successfully synthesized using β-cyclodextrin as the functional monomer because its appropriate size, hydrophobic cavities, and hydroxyl groups benefitted anchoring TRF through noncovalent interactions. An appropriate hydrophilic imprinted layer was generated by self-copolymerization of dopamine and m-aminophenylboronic acid (APBA). After TRF was captured by Fe3O4@Au@cyclodextrin-MIPs, it was labeled with 4-mercaptophenylboronic acid-modified AgNPs as SERS nanotags to construct a sandwich structured sensor. This MMIP-SERS sensor behaved excellent identification and detection performances toward TRF since Fe3O4@Au@cyclodextrin-MIPs contributed to high specificity of the assay and SERS provided high sensitivity. Furthermore, the reliability and feasibility of the sensor in real serum samples were verified by determining TRF from liver disease patients, suggesting its great potential in clinical TRF detection.
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