MOF-derivated MnO@C nanocomposite with bidirectional electrocatalytic ability as signal amplification for dual-signal electrochemical sensing of cancer biomarker

Dual-signal strategy has great potential in improving the accuracy and sensitivity of cancer biomarker determination. However, most sensors based on nanomaterials as signal amplification usually output single detectable signal. It is still a challenge to achieve dual-signal sensing of biomarkers with nanomaterials as signal amplification. Herein, MnO@C nanocomposite was prepared with Mn-MOF-74 as precursor by pyrolysis. It possesses bidirectional electrocatalytic ability toward both oxidation and reduction of H2O2 for its fully exposed crystal facets. After loading AuNPs, MnO@C@AuNPs can connect aptamer (Apt) via Au-S and then as a signal amplification for the construction of sandwich-type aptasensor for dual-signal electrochemical sensing of cancer biomarker. Thus, taking mucin 1 (MUC1) as a model system. The aptasensor has the parallel output of differential pulse voltammetry (DPV) and chronoamperometry responses based on oxidation and reduction of H2O2, respectively, which implemented sensitive and accurate measurements to avoid false results. The linear response ranges of 0.001 nM-100 nM (detection limit of 0.31 pM) for DPV technique and 0.001 nM-10 nM (detection limit of 0.25 pM) for chronoamperometry technique were obtained. It opens up a new way to design elegant dual-signal aptasensors with potential applications in early disease diagnosis.