过滤(数学)
计算流体力学
放大
生物过程
停留时间分布
滤波器(信号处理)
比例(比率)
工艺工程
停留时间(流体动力学)
流体力学
环境科学
流量(数学)
计算机科学
机械
工程类
数学
化学工程
物理
量子力学
经典力学
统计
岩土工程
计算机视觉
作者
Negin Nejatishahidein,Minyoung Kim,Seon Yeop Jung,Ehsan Espah Borujeni,Lara Fernandez‐Cerezo,David J. Roush,Ali Borhan,Andrew L. Zydney
摘要
Abstract Significant increases in cell density and product titer have led to renewed interest in the application of depth filtration for initial clarification of cell culture fluid in antibody production. The performance of these depth filters will depend on the local pressure and velocity distribution within the filter capsule, but these are very difficult to probe experimentally, leading to challenges in both process design and scale‐up. We have used a combination of carefully designed experimental studies and computational fluid dynamics (CFD) to examine these issues in both lab‐scale (Supracap TM 50) and pilot‐scale (Stax TM ) depth filter modules, both employing dual‐layer lenticular PDH4 media containing diatomaceous earth. The Supracap TM 50 showed a more rapid increase in transmembrane pressure and a more rapid DNA breakthrough during filtration of a Chinese Hamster Ovary cell culture fluid. These results were explained using CFD calculations which showed very different flow distributions within the modules. CFD predictions were further validated using measurements of the residence time distribution and dye binding in both the lab‐scale and pilot‐plant modules. These results provide important insights into the factors controlling the performance and scale‐up of these commercially important depth filters as well as a framework that can be broadly applied to develop more effective depth filters and depth filtration processes.
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