低温电子层析成像
低温电子显微
结构生物学
电子断层摄影术
纳米技术
电子显微镜
桥接(联网)
生物物理学
生物
断层摄影术
细胞生物学
材料科学
计算机科学
物理
透射电子显微镜
计算机网络
光学
扫描透射电子显微镜
作者
L. C. T. Young,Elizabeth Villa
标识
DOI:10.1146/annurev-biophys-111622-091327
摘要
Recent advances in cryo-electron microscopy have marked only the beginning of the potential of this technique. To bring structure into cell biology, the modality of cryo-electron tomography has fast developed into a bona fide in situ structural biology technique where structures are determined in their native environment, the cell. Nearly every step of the cryo-focused ion beam-assisted electron tomography (cryo-FIB-ET) workflow has been improved upon in the past decade, since the first windows were carved into cells, unveiling macromolecular networks in near-native conditions. By bridging structural and cell biology, cryo-FIB-ET is advancing our understanding of structure–function relationships in their native environment and becoming a tool for discovering new biology.
科研通智能强力驱动
Strongly Powered by AbleSci AI