生物
细胞生物学
生物发生
线粒体生物发生
干细胞
造血干细胞
造血
细胞命运测定
线粒体
生物化学
基因
转录因子
作者
Massimo Bonora,Claudia Morganti,Nick van Gastel,Kyoko Ito,Enrica Calura,Ilaria Zanolla,Letizia Ferroni,Yang Zhang,Yookyung Jung,Gabriele Sales,Paolo Martini,Takahisa Nakamura,Francesco M. Lasorsa,Toren Finkel,Charles P. Lin,Barbara Zavan,Paolo Pinton,Irene Georgakoudi,Chiara Romualdi,David T. Scadden,Keisuke Ito
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2024-03-01
卷期号:31 (3): 359-377.e10
被引量:7
标识
DOI:10.1016/j.stem.2024.02.004
摘要
Summary
Mitochondrial fatty acid oxidation (FAO) is essential for hematopoietic stem cell (HSC) self-renewal; however, the mechanism by which mitochondrial metabolism controls HSC fate remains unknown. Here, we show that within the hematopoietic lineage, HSCs have the largest mitochondrial NADPH pools, which are required for proper HSC cell fate and homeostasis. Bioinformatic analysis of the HSC transcriptome, biochemical assays, and genetic inactivation of FAO all indicate that FAO-generated NADPH fuels cholesterol synthesis in HSCs. Interference with FAO disturbs the segregation of mitochondrial NADPH toward corresponding daughter cells upon single HSC division. Importantly, we have found that the FAO-NADPH-cholesterol axis drives extracellular vesicle (EV) biogenesis and release in HSCs, while inhibition of EV signaling impairs HSC self-renewal. These data reveal the existence of a mitochondrial NADPH-cholesterol axis for EV biogenesis that is required for hematopoietic homeostasis and highlight the non-stochastic nature of HSC fate determination.
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