乐果
比色法
化学
检出限
漆酶
色谱法
酶分析
酶
生物化学
生物
农学
杀虫剂
作者
Lian Xia,Jianxun Han,Xiangyong Huang,Xiaojuan Niu,Xin Lin,Yuangen Wu
出处
期刊:Food Control
[Elsevier]
日期:2024-04-01
卷期号:158: 110252-110252
被引量:4
标识
DOI:10.1016/j.foodcont.2023.110252
摘要
The overutilization of dimethoate may lead to its deposition in both the environment and crops, which potentially induces neurological, respiratory, and hepatic abnormalities upon absorption by organisms. Colorimetry can accurately determine dimethoate, but there are still many challenges in the rapid, convenient, and point-of-care detection. Herein, based on the inhibitory effect of dimethoate on the laccase activity of silver citrate (AgCit), a smartphone-assisted colorimetric sensor and rapid detection enzyme sheets were constructed. It was demonstrated that dimethoate could adsorb on the surface of AgCit and inhibit the production of semiquinone radicals in the reaction system. Then, the AgCit laccase activity was inhibited, causing the colorimetry sensor and the enzyme sheets turned light red or even colorless. The constructed sensor has higher sensitivity and the limit of detection for dimethoate is 1.0 μg⋅L−1. Moreover, the developed enzyme sheets have good selectivity and specificity, and display the detection results within 5 min. Thus, the colorimetric sensor and the constructed enzyme sheets are expected to be used for large-scale preliminary detection of dimethoate residues in actual agricultural products.
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