结肠炎
炎症性肠病
协议(科学)
疾病
病态的
计算机科学
医学
免疫学
病理
替代医学
作者
Zhen-Ping Zou,Yuhang Fan,Yue Du,Ting-Ting Fang,Yong Du,Ying Zhou,Bang‐Ce Ye
出处
期刊:STAR protocols
[Elsevier]
日期:2023-06-01
卷期号:4 (2): 102254-102254
标识
DOI:10.1016/j.xpro.2023.102254
摘要
Engineered microorganisms hold potential for disease diagnosis and treatment. Here, we present a protocol to engineer E. coli Nissle 1917 strain (iROBOT) using genome insertion and plasmid construction to diagnose, record, and ameliorate inflammatory bowel disease in mice. We describe steps for constructing and administering iROBOT, diagnosing and recording colitis, preparing samples, and analyzing fluorescence and base editing ratios of iROBOT. We detail a colitis ameliorating assay using the disease activity index, colon length, tissue pathological section, and cytokine analysis. For complete details of the use and execution of this protocol, please refer to Zou et al.1.
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