电化学发光
发光
肌钙蛋白I
化学发光
电极
发光测量
化学
分析化学(期刊)
材料科学
核化学
光电子学
物理化学
有机化学
医学
心脏病学
心肌梗塞
作者
Arathy B. Kala,Greeshma Rajeevan,Anju S. Madanan,Susan Varghese,Merin K. Abraham,Ali Ibrahim Shkhair,Geneva Indongo,Sony George
出处
期刊:ACS applied bio materials
[American Chemical Society]
日期:2024-11-01
标识
DOI:10.1021/acsabm.4c01247
摘要
An economical, rapid, and ultrasensitive detection of biomolecules in clinical settings is very crucial, particularly for the early detection of Cardiac Troponin I (cTnI), which is the gold standard biomarker for Acute Myocardial Infarction (AMI). Electrochemiluminescence (ECL) has risen in prominence as an important technique for in vitro diagnosis and detection by virtue of its high sensitivity reaching a femtomolar level. This study introduces an economically feasible nanoplatform for ECL immunosensing, consisting of a gold nanoparticle (AuNP) with Ru(bpy)32+ and tripropylamine (TPA) system, which is a potential ECL luminophore and coreactant system. AuNPs serve the role of an ECL signal enhancer as well as the carrier of antibody, which enables the creation of a label-free immunosensor for antigen–antibody interactions. The prepared immunosensor detected cTnI with a detection limit (LOD) of 0.03 ng/mL. This potential immunosensor provides appreciable results in the detection of cTnI from spiked real serum analysis, which shows its potential application in low-resource clinical settings.
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