Inhibition effect of marine active peptides SF on dual-species biofilms of Vibrio parahaemolyticus and Aeromonas sobria

副溶血性弧菌 群体感应 生物膜 微生物学 胞外聚合物 细胞外 化学 高丝氨酸 生物化学 毒力 生物 铜绿假单胞菌 细菌 基因 遗传学
作者
Xinran Lv,Wenqi Yang,Shuilin Liu,Xueqing Liu,Shuo Yuan,Le An,Anqi Ren,Fengling Bai,Jianrong Li,Xuepeng Li,Yuqiong Meng,Rui Ma
出处
期刊:Food bioscience [Elsevier BV]
卷期号:61: 104697-104697 被引量:1
标识
DOI:10.1016/j.fbio.2024.104697
摘要

To mitigate food contamination caused by multi-species biofilms, the inhibitory effect of peptide SF on dual-species biofilms was investigated in the study. Vibrio parahaemolyticus frequently engages in intercommunication with other bacteria through the autoinducer-2 (AI-2)/LuxS quorum sensing system, contributing to the biofilm formation. However, the knowledge of quorum sensing inhibitors (QSIs) that target dual-species biofilms involving V. parahaemolyticus has remained limited. Therefore, this study constructed dual-species biofilms of V. parahaemolyticus and Aeromonas sobria, assessed AI-2 activity, and investigated the inhibitory effect of the QSI peptide Ser-Phe (SF) on these biofilms. The results indicated that peptide SF (1 mg/mL) significantly inhibited AI-2 activity, production of extracellular polysaccharides, proteins, DNA, metabolic activity, hydrophobicity, and auto-aggregation of these biofilms. The inhibitory rates to the both dual-species and V. parahaemolyticus mono-species were 44.40% and 47.31% for AI-2 activity, 31.29% and 63.84% for extracellular polysaccharides, 53.64% and 48.61% for extracellular proteins, 55.38% and 52.58% for extracellular DNA, 46.18% and 24.46% for metabolic activity, 67.46% and 50.61% for hydrophobicity, and 44.15% and 51.98% for auto-aggregation. Moreover, scanning electron microscopy revealed the disruption of the structure in dual-species biofilms treated with peptide SF. The qRT-PCR results demonstrated that peptide SF down-regulated the expression of key genes related to quorum sensing (luxS and luxP), flagellum (flaA), virulence factors (toxR), and extracellular polymeric substances (cpsA) in the dual-species. However, peptide SF did not inhibit AI-2 activity or biofilm formation in A. sobria. This study will provide a QSI for controlling multi-species biofilms caused by V. parahaemolyticus.
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