Extracellular vesicles miR‐31‐5p promotes pancreatic cancer chemoresistance via regulating LATS2‐Hippo pathway and promoting SPARC secretion from pancreatic stellate cells

胰腺癌 癌症研究 间质细胞 肝星状细胞 河马信号通路 肿瘤微环境 生物 癌细胞 微泡 MAPK/ERK通路 激酶 吉西他滨 细胞生物学 癌症 小RNA 内分泌学 生物化学 基因 遗传学 肿瘤细胞
作者
Cheng Qin,Bangbo Zhao,Yuanyang Wang,Zeru Li,Tianyu Li,Yutong Zhao,Weibin Wang,Yupei Zhao
出处
期刊:Journal of extracellular vesicles [Taylor & Francis]
卷期号:13 (8) 被引量:4
标识
DOI:10.1002/jev2.12488
摘要

Abstract Pancreatic cancer remains one of the most lethal malignant diseases. Gemcitabine‐based chemotherapy is still one of the first‐line systemic treatments, but chemoresistance occurs in the majority of patients. Recently, accumulated evidence has demonstrated the role of the tumour microenvironment in promoting chemoresistance. In the tumour microenvironment, pancreatic stellate cells (PSCs) are among the main cellular components, and extracellular vesicles (EVs) are common mediators of cell‒cell communication. In this study, we showed that SP1‐transcribed miR‐31‐5p not only targeted LATS2 in pancreatic cancer cells but also regulated the Hippo pathway in PSCs through EV transfer. Consequently, PSCs synthesized and secreted protein acidic and rich in cysteins (SPARC), which was preferentially expressed in stromal cells, stimulating Extracellular Signal regulated kinase (ERK) signalling in pancreatic cancer cells. Therefore, pancreatic cancer cell survival and chemoresistance were improved due to both the intrinsic Hippo pathway regulated by miR‐31‐5p and external SPARC‐induced ERK signalling. In mouse models, miR‐31‐5p overexpression in pancreatic cancer cells promoted the chemoresistance of coinjected xenografts. In a tissue microarray, pancreatic cancer patients with higher miR‐31‐5p expression had shorter overall survival. Therefore, miR‐31‐5p regulates the Hippo pathway in multiple cell types within the tumour microenvironment via EVs, ultimately contributing to the chemoresistance of pancreatic cancer cells.
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