分析物
胶体金
生物技术
化学
色谱法
纳米技术
生化工程
纳米颗粒
材料科学
生物
工程类
作者
Pablo Llano‐Suárez,Adrián Sánchez-Visedo,Inmaculada Ortiz‐Gómez,Marı́a Teresa Fernández-Argüelles,Marta Prado,José M. Costa‐Fernández,Ana Soldado
出处
期刊:Biosensors
[Multidisciplinary Digital Publishing Institute]
日期:2024-08-03
卷期号:14 (8): 377-377
被引量:1
摘要
Food safety control is a key issue in the food and agriculture industries. For such purposes, developing miniaturized analytical methods is critical for enabling the rapid and sensitive detection of food supplements, allergens, and pollutants. Here, a novel bioanalytical methodology based on DNA-functionalized gold nanoparticles (AuNPs) and colorimetric detection was developed to detect the presence of sesame (a major allergen) through sesame seed DNA as a target, in food samples. The presence of sesame DNA induces controlled nanoparticle aggregation/desegregation, resulting in a color change (from blue to red) proportional to sesame DNA concentration. The incorporation of multicomponent nucleic acid enzymes (MNAzymes) in this strategy has been carried out to perform an isothermal signal amplification strategy to improve the sensitivity of detection. Also, open-source software for color analysis was used to ensure an unbiased visual color-change detection, enhancing detection accuracy and sensitivity and opening the possibility of performing a simple and decentralized analyte detection. The method successfully detected the presence of sesame DNA in sesame seed, sesame oil, olive oil, and sunflower oil. In brief, the developed approach constitutes a simple and affordable alternative to perform a highly sensitive detection of DNA in food without complex methodologies or the requirement of expensive instrumentation.
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