Cox1 -based phylogeny of Eastern Palearctic Drunella (Ephemeroptera: Ephemerellidae), description of new species and redescription of D. cryptomeria (Imanishi)

The diversity of a mayfly genus, Drunella Needham (Ephemeroptera: Ephemerellidae), was examined using relationships of the barcode region of the cytochrome c oxidase subunit I (cox1) gene. Two novel species, Drunella campicola Ishiwata sp. nov. and Drunella parvicarnivora Ishiwata sp. nov. were described and Drunella cryptomeria was redescribed. A cox1 dendrogram based on the maximum likelihood method consisted of 10 clusters of DNA sequences sampled in Japan, which were roughly divided into three groups. The first group comprised D. cryptomeria and the two newly described species (D. campicola and D. parvicarnivora), the second group included D. basalis and D. ishiyamana, and the third group comprised D. trispina, D. triacantha, D. kohnoi, and D. sachalinensis. A cryptic lineage of D. trispina was observed and the distance between the two D. trispina clades is comparable to that between D. triacantha and each of the two D. trispina clades. The inheritance of the mitochondrial genome in these species is yet unclear, and therefore needs to be studied further. In the present study, we were able to confirm the distinct lineages based on cox1 and aided in the understanding of the taxonomy of the genus Drunella with descriptions of two new species. http://zoobank.org/urn:lsid:zoobank.org:pub:263306F7-A839-4BE6-8252-58080A4524AA