质谱成像
马尔迪成像
质谱法
分析物
基质辅助激光解吸/电离
样品制备
化学
计算机科学
计算生物学
色谱法
数据科学
生化工程
纳米技术
解吸
材料科学
生物
工程类
有机化学
吸附
作者
Jessica L. Moore,Georgia Charkoftaki
标识
DOI:10.1021/acs.jproteome.3c00167
摘要
Imaging mass spectrometry is a well-established technology that can easily and succinctly communicate the spatial localization of molecules within samples. This review communicates the recent advances in the field, with a specific focus on matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) applied on tissues. The general sample preparation strategies for different analyte classes are explored, including special considerations for sample types (fresh frozen or formalin-fixed,) strategies for various analytes (lipids, metabolites, proteins, peptides, and glycans) and how multimodal imaging strategies can leverage the strengths of each approach is mentioned. This work explores appropriate experimental design approaches and standardization of processes needed for successful studies, as well as the various data analysis platforms available to analyze data and their strengths. The review concludes with applications of imaging mass spectrometry in various fields, with a focus on medical research, and some examples from plant biology and microbe metabolism are mentioned, to illustrate the breadth and depth of MALDI IMS.
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