梨
生物
细胞生长
细胞生物学
转录调控
转基因
转录组
单性结实
酵母
植物生理学
细胞分裂素
调节器
转录因子
植物
基因
生物化学
基因表达
生长素
作者
Huibin Wang,Guangya Sha,Rui Gao,Jianwen Pang,Rui Zhai,Chengquan Yang,Zhigang Wang,Lingfei Xu
标识
DOI:10.1016/j.hpj.2022.09.011
摘要
As a cell proliferation regulator involved in wide biological processes in plants, GRF-INTERACTING FACTOR (GIF) controls different tissues development. However, whether GIF participates in fruit development remains unclear. According to transcriptome data, we identified PbGIF1 was highly expressed during fruit development in cytokinins induced parthenocarpy pear. In the present study, the biofunction of PbGIF1 was initially verified. Overexpression of PbGIF1 promoted fruit size of transgenic tomato. The size of flesh fruit was not affected by cell expansion but the cell proliferation was promoted by overexpressing PbGIF1. The accelerated cell proliferation process was also observed in PbGIF1-overexpressed transgenic pear fruit calli. The transcriptional regulation of cytokinins on PbGIF1 was further confirmed by exogenous CPPU treatments in pear fruitlets. To investigate the underlying mechanism, the cytokinins-responded factor, PbRR1, was further focused on. The results of Yeast-one-hybrid assay suggested that PbRR1 can bind to the promoter sequence of PbGIF1. The transcriptional activation of PbRR1 on PbGIF1 was also confirmed by Dual-Luciferase assays. Taken together, the results showed that cytokinins control pear fruit development via the transcriptional activation of PbGIF1 by PbRR1.
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