荧光
背景(考古学)
材料科学
罗丹明
钙黄绿素
荧光素
基质(水族馆)
生物相容性
纳米技术
化学工程
光学
工程类
古生物学
海洋学
物理
冶金
生物
地质学
作者
Patrick C. Hall,Harrison W. Reid,Ievgenii Liashenko,Biranche Tandon,Kelly L. O'Neill,Naomi C. Paxton,Gabriella Lindberg,Ramesh Jasti,Paul D. Dalton
出处
期刊:Small
[Wiley]
日期:2024-06-06
被引量:1
标识
DOI:10.1002/smll.202400882
摘要
Abstract Fluorescent probes are an indispensable tool in the realm of bioimaging technologies, providing valuable insights into the assessment of biomaterial integrity and structural properties. However, incorporating fluorophores into scaffolds made from melt electrowriting (MEW) poses a challenge due to the sustained, elevated temperatures that this processing technique requires. In this context, [n]cycloparaphenylenes ([n]CPPs) serve as excellent fluorophores for MEW processing with the additional benefit of customizable emissions profiles with the same excitation wavelength. Three fluorescent blends are used with distinct [n]CPPs with emission wavelengths of either 466, 494, or 533 nm, identifying 0.01 wt% as the preferred concentration. It is discovered that [n]CPPs disperse well within poly(ε‐caprolactone) (PCL) and maintain their fluorescence even after a week of continuous heating at 80 °C. The [n]CPP‐PCL blends show no cytotoxicity and support counterstaining with commonly used DAPI (Ex/Em: 359 nm/457 nm), rhodamine‐ (Ex/Em: 542/565 nm), and fluorescein‐tagged (Ex/Em: 490/515 nm) phalloidin stains. Using different color [n]CPP‐PCL blends, different MEW fibers are sequentially deposited into a semi‐woven scaffold and onto a solution electrospun membrane composed of [8]CPP‐PCL as a contrasting substrate for the [10]CPP‐PCL MEW fibers. In general, [n]CPPs are potent fluorophores for MEW, providing new imaging options for this technology.
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