吲哚青绿
光热治疗
活性氧
氧化应激
生物物理学
氧化磷酸化
激进的
体内
化学
热休克蛋白27
体外
线粒体
热休克蛋白
羟基自由基
热休克蛋白70
材料科学
纳米技术
生物化学
外科
生物
生物技术
基因
医学
作者
Xin Ding,Tianyu Wang,Shiwei Bai,Mian Yang,Na Peng,Tao Qiu,Yi Liu
标识
DOI:10.1002/adhm.202300929
摘要
Abstract The purpose of this study is to down‐regulate heat shock proteins and improve the mild photothermal therapy (mild‐PTT) effect of polydopamine (PDA) by preparing the nanosystem of Cu 2+ and indocyanine green (ICG)–loaded PDA nanospheres with surface modification of integrin‐targeted cyclic peptide (cRGD) (PDA/Cu/ICG/R), which can limit ATP synthesis through the double mitochondrial destruction pathway. In vitro and in vivo experiments using PDA/Cu/ICG/R irradiated with an NIR laser demonstrate that when NIR is “OFF,” Cu 2+ can undergo Fenton‐like reaction in tumor cells, producing a large amount of hydroxyl radicals (·OH), which leads to oxidative stress in cells. This oxidative stress can cause mitochondrial oxidative phosphorylation dysfunction, resulting in limited ATP synthesis. When NIR is “ON,” mild‐PTT can accelerate Cu 2+ to produce ·OH. Simultaneously, NIR can activate ICG to produce reactive oxygen species (ROS) storm, amplify intracellular oxidative stress, and continuously damage mitochondria. The biodegradability of PDA greatly reduces the risk of toxicity caused by long‐term retention of PDA/Cu/ICG/R in organisms. Finally, the improvement of the mild‐PTT effect of PDA is successfully achieved through the double mitochondrial destruction pathway of Cu 2+ and ICG controlled by NIR “switch.”
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