Regulation of Pre-Osteoblast Osteogenic Transcription Factors by Different Titanium Surface Topography

The aim of the present study is to verify the morphogenic change and expression of Runx-2, Osterix, and osteocalcin (OCN) of pre-osteoblasts on the titanium surfaces with machine polished process and MAO.The results indicate that the roughness, instead of the variant of methodology for metal surface conditioning, determines the response of MC3T3-E1 cells and the gene expression of Runx-2, Osterix, and OCN.The highest expression of Runx-2 and Osterix in smooth surface group happened late after 72 hours of incubation implied that the pre-osteoblasts showed a delay response of preosteogenesis on shining titanium surface compared to that of MAO group.The 1μm group showed the utmost expression of OCN as compared to those of smooth surface group and the two others groups.It can be concluded that roughness of 1μm may be the more preferred surface architecture for pre-osteoblasts to evolute and differentiate into osteoblasts, hence accelerate the process of osteogenesis.