Immune regulatory effects of FTY720 on Dendritic cells (P1.142)

格里芬 免疫系统 心理学 医学 神经科学 免疫学 艺术 经典
作者
Maria Antonietta Mazzola,Lily Aly,Russell Griffin,Parham Nejad,Howard L. Weiner,Roopali Gandhi
出处
期刊:Neurology [Lippincott Williams & Wilkins]
卷期号:84 (14_supplement)
标识
DOI:10.1212/wnl.84.14_supplement.p1.142
摘要

Objective: To determine the immune regulatory effects of FTY720 on Dendritic cells (DCs). Background: Dendritic cells (DCs) control the activation and differentiation of myelin-specific effector T lymphocytes and regulatory T-cells. They play a pivotal role in CNS autoimmunity and inflammatory responses. DCs are the primary cells involved in T cell education and thus significantly contribute to control the T cell mediated immunity and tolerance. FTY720 is the functional analogue of Sphingosine and is the first oral treatment for relapsing remitting Multiple Sclerosis (MS). FTY720 regulates immune cell trafficking and prevents relapses in Multiple Sclerosis. We investigated immune regulatory effects of FTY720 on dendritic cells. Method: The percentages and phenotype of immune cells in longitudinal samples from MS patients were studied by FACs (flourscence activated cell staining) analysis. DCs were purified using miltenyi bead sorting and cultured overnight in the presence or absence of FTY720. Activated DCs were co-cultured with T cells to determine the effect of FTY720 treated DCs on T cell differentiation. T cell proliferation was measured by thymidine incorporation and cytokines produced by the activated cells were measured using luminex assay. Results: We found that the percentages and the absolute numbers of total T and B cells were decreased in MS patients treated with FTY720. Interestingly, we found that the percentages and the absolute numbers of myeloid DCs were increased in the peripheral blood from FTY720 treated patients. We did not find any differences in the activation and costimulatory markers expressed by DCs before and after FTY720 treatment. We found that the DCs cultured in the presence of FTY720 have tolerogenic phenotype as T cells cultured with FTY720 treated DCS have decreased proliferation and decreased cytokine production. Conclusion: FTY720 induces tolerogenic DCs that induce less inflammatory T cell responses.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
利利完成签到,获得积分10
刚刚
刚刚
李爱国应助yuer采纳,获得10
1秒前
1秒前
LDD关闭了LDD文献求助
1秒前
李健的小迷弟应助111采纳,获得10
1秒前
英姑应助WWWWH采纳,获得10
2秒前
风吹不到海湾完成签到,获得积分10
2秒前
March应助wwrjj采纳,获得10
2秒前
SciGPT应助开放的寒梅采纳,获得10
2秒前
3秒前
漂亮的笑萍完成签到,获得积分10
4秒前
可爱柠檬完成签到,获得积分10
4秒前
4秒前
等待蚂蚁完成签到 ,获得积分10
5秒前
chen完成签到,获得积分10
5秒前
ZHDNCG发布了新的文献求助10
5秒前
123123发布了新的文献求助10
6秒前
慕青应助0713采纳,获得10
6秒前
Even发布了新的文献求助10
6秒前
7秒前
7秒前
打打应助winwin采纳,获得10
7秒前
8秒前
8秒前
汉堡包应助美满花生采纳,获得10
8秒前
8秒前
黄腾完成签到,获得积分10
9秒前
搜索v完成签到 ,获得积分10
9秒前
9秒前
9秒前
10秒前
11秒前
李健应助naomi采纳,获得10
12秒前
Andy1201完成签到,获得积分10
12秒前
林小不脏完成签到,获得积分10
12秒前
enen发布了新的文献求助30
12秒前
greenspanee发布了新的文献求助10
12秒前
12秒前
12秒前
高分求助中
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Effective Learning and Mental Wellbeing 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3974426
求助须知:如何正确求助?哪些是违规求助? 3518788
关于积分的说明 11195842
捐赠科研通 3254946
什么是DOI,文献DOI怎么找? 1797649
邀请新用户注册赠送积分活动 877037
科研通“疑难数据库(出版商)”最低求助积分说明 806130