悬臂梁
单层
吸附
缓冲器(光纤)
材料科学
病毒
图层(电子)
硅
化学
光电子学
纳米技术
分析化学(期刊)
色谱法
病毒学
复合材料
生物
电信
有机化学
计算机科学
作者
B. Ilic,Yi Yang,Harold G. Craighead
摘要
We have used a resonating mechanical cantilever to detect immunospecific binding of viruses, captured from liquid. As a model virus, we used a nonpathogenic insect baculovirus to test the ability to specifically bind and detect small numbers of virus particles. Arrays of surface micromachined, antibody-coated polycrystalline silicon nanomechanical cantilever beams were used to detect binding from various concentrations of baculoviruses in a buffer solution. Because of their small mass, the 0.5μm×6μm cantilevers have mass sensitivities on the order of 10−19g∕Hz, enabling the detection of an immobilized AcV1 antibody monolayer corresponding to a mass of about 3×10−15g. With these devices, we can detect the mass of single-virus particles bound to the cantilever. Resonant frequency shift resulting from the adsorbed mass of the virus particles distinguished solutions of virus concentrations varying between 105 and 107pfu∕ml. Control experiments using buffer solutions without baculovirus showed small amounts (<50attograms) of nonspecific adsorption to the antibody layer.
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