Bioreactor scale-up and oxygen transfer rate in microbial processes: An overview

生物反应器 传质 生物过程 混合(物理) 连续搅拌釜式反应器 氧气 传质系数 化学 气泡 化学需氧量 鼓泡塔反应器 化学工程 体积流量 放大 废水 制浆造纸工业 色谱法 环境工程 环境科学 热力学 机械 气泡 有机化学 物理化学 工程类 物理 经典力学 量子力学
作者
Félix Garcı́a-Ochoa,Emilio Gómez
出处
期刊:Biotechnology Advances [Elsevier BV]
卷期号:27 (2): 153-176 被引量:1287
标识
DOI:10.1016/j.biotechadv.2008.10.006
摘要

In aerobic bioprocesses, oxygen is a key substrate; due to its low solubility in broths (aqueous solutions), a continuous supply is needed. The oxygen transfer rate (OTR) must be known, and if possible predicted to achieve an optimum design operation and scale-up of bioreactors. Many studies have been conducted to enhance the efficiency of oxygen transfer. The dissolved oxygen concentration in a suspension of aerobic microorganisms depends on the rate of oxygen transfer from the gas phase to the liquid, on the rate at which oxygen is transported into the cells (where it is consumed), and on the oxygen uptake rate (OUR) by the microorganism for growth, maintenance and production. The gas-liquid mass transfer in a bioprocess is strongly influenced by the hydrodynamic conditions in the bioreactors. These conditions are known to be a function of energy dissipation that depends on the operational conditions, the physicochemical properties of the culture, the geometrical parameters of the bioreactor and also on the presence of oxygen consuming cells. Stirred tank and bubble column (of various types) bioreactors are widely used in a large variety of bioprocesses (such as aerobic fermentation and biological wastewater treatments, among others). Stirred tanks bioreactors provide high values of mass and heat transfer rates and excellent mixing. In these systems, a high number of variables affect the mass transfer and mixing, but the most important among them are stirrer speed, type and number of stirrers and gas flow rate used. In bubble columns and airlifts, the low-shear environment compared to the stirred tanks has enabled successful cultivation of shear sensitive and filamentous cells. Oxygen transfer is often the rate-limiting step in the aerobic bioprocess due to the low solubility of oxygen in the medium. The correct measurement and/or prediction of the volumetric mass transfer coefficient, (k(L)a), is a crucial step in the design, operation and scale-up of bioreactors. The present work is aimed at the reviewing of the oxygen transfer rate (OTR) in bioprocesses to provide a better knowledge about the selection, design, scale-up and development of bioreactors. First, the most used measuring methods are revised; then the main empirical equations, including those using dimensionless numbers, are considered. The possible increasing on OTR due to the oxygen consumption by the cells is taken into account through the use of the biological enhancement factor. Theoretical predictions of both the volumetric mass transfer coefficient and the enhancement factor that have been recently proposed are described; finally, different criteria for bioreactor scale-up are considered in the light of the influence of OTR and OUR affecting the dissolved oxygen concentration in real bioprocess.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
WeiBao发布了新的文献求助10
刚刚
水清木华完成签到,获得积分10
刚刚
曹国庆完成签到 ,获得积分10
1秒前
1秒前
莫愁发布了新的文献求助10
1秒前
2秒前
chen完成签到,获得积分10
2秒前
2秒前
kjbt完成签到,获得积分20
2秒前
冯家源完成签到,获得积分10
2秒前
3秒前
仿真小学生完成签到,获得积分10
3秒前
ll应助fuguier采纳,获得10
3秒前
旺仔完成签到,获得积分10
4秒前
英俊延恶完成签到,获得积分10
4秒前
4秒前
syjjj完成签到,获得积分10
4秒前
斯文以蓝完成签到,获得积分10
4秒前
江舟添盛望完成签到 ,获得积分10
4秒前
Cuddle完成签到 ,获得积分10
5秒前
勤劳宛菡完成签到 ,获得积分10
5秒前
6秒前
望望旺仔牛奶完成签到,获得积分10
6秒前
GL发布了新的文献求助10
7秒前
杜嘟嘟完成签到,获得积分10
7秒前
Rick完成签到,获得积分10
7秒前
7秒前
斯文败类应助溪水采纳,获得10
7秒前
龙江游侠完成签到,获得积分10
8秒前
8秒前
8秒前
宝贝完成签到,获得积分10
9秒前
轻狂书生完成签到,获得积分10
9秒前
kjbt发布了新的文献求助10
9秒前
xxx_oo完成签到,获得积分10
12秒前
科研通AI2S应助老八的嘴采纳,获得10
12秒前
充电宝应助古炮采纳,获得10
12秒前
乖猫要努力完成签到,获得积分10
14秒前
iceeer完成签到,获得积分10
14秒前
Fawn完成签到 ,获得积分10
16秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968603
求助须知:如何正确求助?哪些是违规求助? 3513420
关于积分的说明 11168029
捐赠科研通 3248900
什么是DOI,文献DOI怎么找? 1794540
邀请新用户注册赠送积分活动 875187
科研通“疑难数据库(出版商)”最低求助积分说明 804676