动态光散射
水动力半径
粘度
聚苯乙烯
光散射
甘油
吞吐量
材料科学
半径
散射
化学
分析化学(期刊)
色谱法
纳米技术
光学
聚合物
计算机科学
物理
纳米颗粒
生物化学
复合材料
电信
无线
计算机安全
作者
Feng He,Gerald W. Becker,Jennifer R. Litowski,Linda O. Narhi,David N. Brems,Vladimir I. Razinkov
标识
DOI:10.1016/j.ab.2009.12.003
摘要
We propose a new method to measure the viscosity of concentrated protein solutions in a high-throughput format. This method measures the apparent hydrodynamic radius of polystyrene beads with known sizes using a dynamic light scattering (DLS) system with a microplate reader. Glycerol solution viscosities obtained by the DLS method were in good agreement with those reported in the literature. Viscosity of the solutions of two monoclonal antibody molecules was acquired using both DLS and cone-and-plate techniques, and the results were comparable. The DLS method described here has the potential to be used in many aspects of protein characterization.
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