pmoA Primers for Detection of Anaerobic Methanotrophs

ABSTRACT Published pmoA primers do not match the pmoA sequence of “ Candidatus Methylomirabilis oxyfera,” a bacterium that performs nitrite-dependent anaerobic methane oxidation. Therefore, new pmoA primers for the detection of “ Ca . Methylomirabilis oxyfera”-like methanotrophs were developed and successfully tested on freshwater samples from different habitats. These primers expand existing molecular tools for the study of methanotrophs in the environment.