乙二醇
生物相容性
材料科学
药物输送
纳米技术
表面改性
石墨烯
纳米载体
纳米材料
PEG比率
共轭体系
紫杉醇
纳米医学
组合化学
化学工程
纳米颗粒
有机化学
化学
聚合物
癌症
医学
财务
经济
内科学
工程类
冶金
复合材料
作者
Zhiyuan Xu,Song Wang,Yongjun Li,Mingwei Wang,Ping Shi,Xiaoyu Huang
摘要
Graphene oxide (GO), a novel 2D nanomaterial prepared by the oxidation of natural graphite, has been paid much attention in the area of drug delivery due to good biocompatibility and low toxicity. In the present work, 6-armed poly(ethylene glycol) was covalently introduced into the surface of GO sheets via a facile amidation process under mild conditions, making the modified GO, GO-PEG (PEG: 65 wt %, size: 50-200 nm), stable and biocompatible in physiological solution. This nanosized GO-PEG was found to be nontoxic to human lung cancer A549 and human breast cancer MCF-7 cells via cell viability assay. Furthermore, paclitaxel (PTX), a widely used cancer chemotherapy drug, was conjugated onto GO-PEG via π-π stacking and hydrophobic interactions to afford a nanocomplex of GO-PEG/PTX with a relatively high loading capacity for PTX (11.2 wt %). This complex could quickly enter into A549 and MCF-7 cells evidenced by inverted fluorescence microscopy using Fluorescein isothiocyanate as a probe, and it also showed remarkably high cytotoxicity to A549 and MCF-7 cells in a broad range of concentration of PTX and time compared to free PTX. This kind of nanoscale drug delivery system on the basis of PEGylated GO may find potential application in biomedicine.
科研通智能强力驱动
Strongly Powered by AbleSci AI