索拉尼镰刀菌
化学
过氧化氢
羟基化
生物化学
丁基羟基甲苯
酶
芘
活性氧
氰化钾
胡椒基丁醇
抗氧化剂
氰化物
有机化学
微生物学
生物
毒性
作者
Étienne Veignie,Catherine Rafin,Patrice Woisel,Fabrice Cazier
标识
DOI:10.1016/j.envpol.2003.11.007
摘要
In order to study the enzymatic mechanisms involved in the successive steps of BaP degradation by a Deuteromycete fungus Fusarium solani, we developed an indirect approach by using inhibitors of enzymes. We used either specific inhibitors of peroxidases (i.e. salicylhydroxamic acid) and of cytochrome P-450 (i.e. piperonyl butoxyde) or inhibitors of both enzymes (i.e. potassium cyanide). Surprisingly, no expected decrease of BaP degradation was observed with most inhibitors tested. On the contrary, more BaP was degraded. Only butylated hydroxytoluene, which acts as a free radical scavenger, inhibited BaP degradation. The inhibition of these enzymes, which use H(2)O(2) as a cosubstrate, might have resulted in an increase of hydrogen peroxide availability in the fungal cultures. This enhancement could induce formation of reactive oxygen species (ROS) which might be the agents that initiate benzo[a]pyrene oxidation. This study proposed a hypothetic alternative metabolic pathway involved in PAH metabolism by Fusarium solani.
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