白腐真菌
黄孢菌
纤维素酶
绿色木霉
化学
水解
木质素
酶水解
发酵
水解物
食品科学
生物化学
有机化学
作者
Lei Zhao,Guangli Cao,Aijie Wang,Hong‐Yu Ren,D. W. Dong,Z.L. Liu,Xiaoyu Guan,Chengjiao Xu,Nanqi Ren
标识
DOI:10.1016/j.biortech.2012.03.076
摘要
The feasibility of fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enzymatic saccharification and H2 production was investigated in this study. Firstly, cornstalk was pretreated with P. chrysosporium at 29 °C under static condition for 15 d, lignin reduction was up to 34.3% with holocellulose loss less than 10%. Microscopic structure observation combined FTIR analysis further demonstrated that the lignin and crystallinity were decreased. Subsequently, the fungal-pretreated cornstalk was subjected to enzymatic hydrolysis by the crude cellulase from Trichoderma viride to produce fermentable sugars which were then fermented to bio-H2 using Thermoanaerobacterium thermosaccharolyticum W16. The maximum enzymatic saccharification was found to be 47.3% which was 20.3% higher than the control without pretreatment. Upon fermentation of enzymatic hydrolysate, the yield of H2 was calculated to be 80.3 ml/g-pretreated cornstalk. The present results suggested the potential of using hydrogen-producing bacteria for high-yield conversion of cornstalk into bio-H2 integrate with biological pretreatment and enzymatic saccharification.
科研通智能强力驱动
Strongly Powered by AbleSci AI