染色
发育生物学
偏振显微镜
显微镜
污渍
细胞生物学
化学
病理
生物
生物医学工程
分子生物学
银染
固定剂
医学
作者
L. C. Junqueira,G. Bignolas,Ricardo R. Brentani
出处
期刊:Histochemical Journal
[Springer Nature]
日期:1979-07-01
卷期号:11 (4): 447-455
被引量:2210
摘要
Sirius Red, a strong anionic dye, stains collagen by reacting, via its sulphonic acid groups, with basic groups present in the collagen molecule. The elongated dye molecules are attached to the collagen fibre in such a way that their long axes are parallel. This parallel relationship between dye and collagen results in an enhanced birefringency. Examination of tissue sections from 15 species of vertebrates suggests that staining with Sirius Red, when combined with enhancement of birefringency, may be considered specific for collagen. An improved and modified method of staining with Sirius Red is presented.
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