Quantitative susceptibility mapping of kidney inflammation and fibrosis in type 1 angiotensin receptor‐deficient mice

纤维化 炎症 病理 血管紧张素II 医学 髓质 磁共振成像 受体 内科学 放射科
作者
Luke Xie,Matthew A. Sparks,Wěi Li,Yi Qi,Chunlei Liu,Thomas M. Coffman,G. Allan Johnson
出处
期刊:NMR in Biomedicine [Wiley]
卷期号:26 (12): 1853-1863 被引量:50
标识
DOI:10.1002/nbm.3039
摘要

Disruption of the regulatory role of the kidneys leads to diverse renal pathologies; one major hallmark is inflammation and fibrosis. Conventional magnitude MRI has been used to study renal pathologies; however, the quantification or even detection of focal lesions caused by inflammation and fibrosis is challenging. We propose that quantitative susceptibility mapping (QSM) may be particularly sensitive for the identification of inflammation and fibrosis. In this study, we applied QSM in a mouse model deficient for angiotensin receptor type 1 (AT 1 ). This model is known for graded pathologies, including focal interstitial fibrosis, cortical inflammation, glomerulocysts and inner medullary hypoplasia. We acquired high‐resolution MRI on kidneys from AT 1 ‐deficient mice that were perfusion fixed with contrast agent. Two MR sequences were used (three‐dimensional spin echo and gradient echo) to produce three image contrasts: T 1 , T 2 * (magnitude) and QSM. T 1 and T 2 * (magnitude) images were acquired to segment major renal structures and to provide landmarks for the focal lesions of inflammation and fibrosis in the three‐dimensional space. The volumes of major renal structures were measured to determine the relationship of the volumes to the degree of renal abnormalities and magnetic susceptibility values. Focal lesions were segmented from QSM images and were found to be closely associated with the major vessels. Susceptibilities were relatively more paramagnetic in wild‐type mice: 1.46 ± 0.36 in the cortex, 2.14 ± 0.94 in the outer medulla and 2.10 ± 2.80 in the inner medulla (10 –2 ppm). Susceptibilities were more diamagnetic in knockout mice: –7.68 ± 4.22 in the cortex, –11.46 ± 2.13 in the outer medulla and –7.57 ± 5.58 in the inner medulla (10 –2 ppm). This result was consistent with the increase in diamagnetic content, e.g. proteins and lipids, associated with inflammation and fibrosis. Focal lesions were validated with conventional histology. QSM was very sensitive in detecting pathology caused by small focal inflammation and fibrosis. QSM offers a new MR contrast mechanism to study this common disease marker in the kidney. Copyright © 2013 John Wiley & Sons, Ltd.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
2秒前
2秒前
Orange应助高高高采纳,获得10
3秒前
曾文治完成签到 ,获得积分10
3秒前
yimuchenlin完成签到,获得积分10
3秒前
ED应助有点鸭梨呀采纳,获得10
4秒前
Darren发布了新的文献求助10
4秒前
冬冬发布了新的文献求助10
4秒前
Lee关闭了Lee文献求助
4秒前
little完成签到,获得积分10
5秒前
啊啊发布了新的文献求助10
6秒前
6秒前
魏少爷发布了新的文献求助10
8秒前
8秒前
chen发布了新的文献求助10
9秒前
猪猪hero应助澡雪采纳,获得10
9秒前
9秒前
慕青应助gu123采纳,获得10
10秒前
大模型应助冬至季采纳,获得10
10秒前
啊啊发布了新的文献求助10
11秒前
科研通AI5应助Qwe采纳,获得10
13秒前
maguodrgon发布了新的文献求助10
13秒前
14秒前
YamDaamCaa应助科研通管家采纳,获得30
14秒前
君君应助科研通管家采纳,获得20
14秒前
Lucas应助科研通管家采纳,获得10
14秒前
大模型应助科研通管家采纳,获得10
14秒前
科研通AI5应助科研通管家采纳,获得30
14秒前
Akim应助科研通管家采纳,获得10
14秒前
慕青应助科研通管家采纳,获得10
14秒前
YamDaamCaa应助科研通管家采纳,获得30
14秒前
共享精神应助科研通管家采纳,获得10
15秒前
15秒前
15秒前
Hello应助科研通管家采纳,获得10
15秒前
Akim应助荷塘月色采纳,获得10
15秒前
16秒前
17秒前
YifanWang应助大胆的夏天采纳,获得10
18秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3975814
求助须知:如何正确求助?哪些是违规求助? 3520123
关于积分的说明 11201020
捐赠科研通 3256502
什么是DOI,文献DOI怎么找? 1798347
邀请新用户注册赠送积分活动 877523
科研通“疑难数据库(出版商)”最低求助积分说明 806417