Quantitative Analysis and Efficient Surface Modification of Silica Nanoparticles

Aminofunctional trialkoxysilanes such as aminopropyltrimethoxysilane (APTMS) and (3‐trimethoxysilylpropyl)diethylenetriamine (DETAS) were employed as a surface modification molecule for generating monolayer modification on the surface of silica (SiO 2 ) nanoparticles. We were able to quantitatively analyze the number of amine functional groups on the modified SiO 2 nanoparticles by acid‐base back titration method and determine the effective number of amine functional groups for the successive chemical reaction by absorption measurements after treating with fluorescent rhodamine B isothiocyanate (RITC) molecules. The numbers of amine sites measured by back titration were 2.7 and 7.7 ea/nm 2 for SiO 2 ‐APTMS and SiO 2 ‐DETAS, respectively, while the numbers of effective amine sites measured by absorption calibration were about one fifth of the total amine sites, namely, 0.44 and 1.3 ea/nm 2 for SiO 2 ‐APTMS(RITC) and SiO 2 ‐DETAS(RITC), respectively. Furthermore, it was confirmed that the reactivity of amino groups on the surface‐modified silica nanoparticles could be maintained in ethanol for more than 1.5 months without showing any significant differences in the reactivity.