Chemical Constituents of the Seeds ofRaphanus sativusand their Biological Activity

As part of our ongoing search for bioactive constituents of natural Korean medicinal resources, a bioassay-guided fractionation and chemical investigation of the MeOH extract of Raphanus sativus (Brassicaceae) seeds resulted in the isolation and identification of fifteen compounds, including a new phenolic compound.The structure of the new compound was determined by extensive spectroscopic analysis and the Mosher's method.One of the compounds has been recently reported as a synthetic product.Some compounds showed moderate antiproliferative activities against the tumor cell lines A549, SK-OV-3, SK-MEL-2, and HCT-15 with IC 50 values in the range of 5.62 to 28.88 μM.Moreover, the anti-neuroinflammatory activities of the isolates were determined by measuring the nitric oxide (NO) levels in the medium using murine microglia BV-2 cells.With exception of one specific compound, all the others inhibited the lipopolysaccharide (LPS)-stimulated NO production (IC 50 values < 200 μM).