哈卡特
DPPH
阿布茨
曲酸
化学
抗氧化剂
皮肤美白
多酚
萃取(化学)
活力测定
生物化学
响应面法
脂质过氧化
细胞毒性
色谱法
食品科学
酪氨酸酶
酶
生物
体外
药理学
活性成分
作者
Huimin Liu,Sheng-Nan Lei,Wei Tang,Meng-Han Xun,Zhi-Wei Zhao,Ming-Yan Cheng,Xiaodan Zhang,Wei Wang
出处
期刊:Molecules
[MDPI AG]
日期:2022-03-16
卷期号:27 (6): 1914-1914
被引量:5
标识
DOI:10.3390/molecules27061914
摘要
In this study, ultrasonic-assisted cellulase extraction (UCE) was applied to extract flavonoids and polyphenols from the Nymphaea hybrid flower. The extraction conditions were optimized using the response surface method (RSM) coupled with a Box-Behnken design. The crude extract of Nymphaea hybrid (NHE) was further purified using AB-8 macroporous resins, and the purified extract (NHEP) was characterized by FTIR and HPLC. In vitro activity determination by chemical method showed that NHEP displayed strong free radical scavenging abilities against the DPPH and ABTS radicals, good reduction power, and hyaluronidase inhibition. The cell viability by CCK-8 assays showed that NHEP had no significant cytotoxicity for B16 and HaCaT cells when the concentration was below 100 μg/mL and 120 μg/mL, respectively. NHEP with a concentration of 20-160 μg/mL can more effectively reduce the ROS level in H2O2 damaged HaCaT cells compared with 10 μg/mL of VC. The 40 μg/mL of NHEP had similar activity against intracellular melanin production in the B16 melanoma cells compared with 20 μg/mL Kojic acid. Good activities of antioxidation, whitening and protective effect against H2O2-induced oxidative damage promote the potential for NHEP as a functional raw material in the field of cosmetics and medicine.
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