A near-infrared plasma membrane-specific AIE probe for fluorescence lifetime imaging of phagocytosis

Abstract Phagocytosis is a biological process that plays a key role in host defense and tissue homeostasis. Efficient approaches for realtime imaging of phagocytosis are highly desired but limited. Herein, an AIE-active near-infrared fluorescent probe, named TBTCP , was developed for fluorescence lifetime imaging of phagocytosis. TBTCP could selectively label the cell plasma membrane with fast staining, wash-free process, high signal-to-background ratio, and excellent photostability. Cellular membrane statuses under different osmolarities as well as macrophage phagocytosis of bacteria or large silica particles in early stages could be reported by the fluorescence lifetime changes of TBTCP . Compared with current fluorescence imaging methods, which target the bioenvironmental changes in the late phagocytosis stage, this approach detects the changes in the cell membrane, thus giving a faster response to phagocytosis. This article provides a functional tool to report the phagocytic dynamics of macrophages which may greatly contribute to the studies of phagocytic function-related diseases.