类胡萝卜素
色谱法
高效液相色谱法
萃取(化学)
化学
生物化学
作者
Maria Sholola,Jessica L. Cooperstone
出处
期刊:Methods in Enzymology
日期:2022-01-01
卷期号:: 423-457
被引量:3
标识
DOI:10.1016/bs.mie.2022.03.021
摘要
Accurate and sensitive methods to quantify carotenoids in blood plasma/serum are the basis for assessing carotenoid intake and associating physiological effects. This chapter introduces carotenoid chemistry, an overview of carotenoid food sources, and current knowledge of carotenoid absorption and metabolism, along with factors that affect these processes. We also detail a commonly used method to extract plasma/serum carotenoids using liquid-liquid extraction and analysis by high performance liquid chromatography coupled with diode array detection (HPLC-DAD). A spreadsheet to aid in this quantitative analysis can be found at www.github.com/CooperstoneLab/carotenoid-analysis.
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