Coherent Raman Scattering with STELLARIS 8 CRS – Applications and Innovations

Coherent Raman Scattering microscopy (CRS) is a powerful approach for label-free, chemically specific imaging, based on the characteristic vibrational contrast of the sample molecules. Here, we introduce the all-new Leica STELLARIS 8 CRS microscope, a multi-modal optical discovery instrument that offers a unique combination of biochemical, biophysical and molecular contrasts. This is achieved by a seamless integration of Stimulated Raman Scattering (SRS), CARS, two-photon fluorescence and second-harmonic generation, with the STELLARIS confocal microscope. We will present applications in neurodegenerative disease, cancer, 3D biology, stem cell and developmental biology, and pharmacology.