Screening of efficient ammonia–nitrogen degrading bacteria and its application in livestock wastewater

The screening of new strains of high-efficiency ammonia–nitrogen degrading bacteria and the research on the treatment of high-concentration ammonia–nitrogen livestock and poultry wastewater by immobilized complex flora is still lacking, especially the research on different ratios between complex flora and operating factors. In this experiment, a new strain of ammonia–nitrogen degrading bacteria was screened from activated sludge. The species was determined by ITS sequencing, and the optimal operating conditions were explored by the simulated wastewater experiment. Build a complex flora with flocculant-producing bacteria preserved in the laboratory. Explore the effects of water temperature, pH, and initial ammonia–nitrogen concentration on the treatment of livestock wastewater with high ammonia–nitrogen by immobilized complex flora. The most similar of this strain is Phialemoniopsis curvata TWCC 58054 s, and the degradation rate of ammonia–nitrogen is 96.90% under the conditions of ammonia–nitrogen concentration of 50.00 mg/L, the water temperature of 30 ℃, pH = 7.2–7.4, and shaking culture for 7 days. At 400.00 mg/L, the water temperature of 30 ℃, pH = 8, shaking culture for 36 h, the degradation rate of ammonia–nitrogen was 57.39%. The optimal ratio of ammonia–nitrogen degrading bacteria to flocculant-producing bacteria is 1:3, and the ammonia–nitrogen degradation rate of immobilized complex bacteria is 85.43–89.84% after shaking culture for 36 h at the influent ammonia–nitrogen concentration of 400.00–500.00 mg/L, the water temperature of 30 ℃ and pH value of 8. The experimental results provided a new type of high-efficiency ammonia–nitrogen degrading bacteria, and described in detail the effects of the ratio of complex bacteria, pH, water temperature, influent concentration, on its treatment of livestock wastewater. It fills the gap of the new strain in ammonia–nitrogen degradation ability and immobilized complex flora.