DNA甲基化
表观遗传学
亚硫酸氢盐测序
生物
计算生物学
表观遗传学
甲基化DNA免疫沉淀
照明菌甲基化试验
甲基化
遗传学
DNA
基因
基因表达
作者
Ting Gong,Heather Borgard,Zao Zhang,Shaoqiu Chen,Zitong Gao,Youping Deng
标识
DOI:10.1002/smtd.202101251
摘要
Abstract DNA methylation is associated with transcriptional repression, genomic imprinting, stem cell differentiation, embryonic development, and inflammation. Aberrant DNA methylation can indicate disease states, including cancer and neurological disorders. Therefore, the prevalence and location of 5‐methylcytosine in the human genome is a topic of interest. Whole‐genome bisulfite sequencing (WGBS) is a high‐throughput method for analyzing DNA methylation. This technique involves library preparation, alignment, and quality control. Advancements in epigenetic technology have led to an increase in DNA methylation studies. This review compares the detailed experimental methodology of WGBS using accessible and up‐to‐date analysis tools. Practical codes for WGBS data processing are included as a general guide to assist progress in DNA methylation studies through a comprehensive case study.
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