抗体
抗原
乳胶固色试验
化学
凝集(生物学)
免疫球蛋白M
色谱法
分子生物学
凝胶电泳
免疫球蛋白G
生物
免疫学
生物化学
作者
Koei Sato,Yayoi Ise,Takashi Iida,Takakazu Suzuki,K Shimada,Kusuya Nishioka
标识
DOI:10.1016/0022-1759(87)90149-9
摘要
Three major components (designated Sp-1, -2 and -3) of the microsomal pellet of Toxoplasma gondii (Tp) were isolated by Ultragel AcA 44 gel filtration chromatography from the microsomal pellet solubilized with detergents. Of these, Sp-2 proved to be most reactive with anti-Tp antibodies and its reactivity with IgM and IgG antibodies varied with the concentration at which it was used for sensitizing latex particles. Sp-2 antigen reacted with IgM antibody alone when latex particles were sensitized with less than or equal to 100 micrograms of this antigen/mg of particles, and its reactivity with IgG antibody appeared and increased progressively with increasing sensitizing concentrations of this antigen. Based on this finding, a method of direct measurement of anti-Tp IgM antibody in serum by passive latex agglutination has been developed. Polyacrylamide disc gel electrophoretic analysis of Sp-2 antigen in the presence of SDS revealed four constituents of 43, 35, 28 and 14 kDa. All these components reached with both IgM and IgG when tested by immunoblotting.
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