Dietary glycerides of valerate ameliorate diarrhea and impact intestinal physiology and serum biomarkers in weaned piglets infected with enterotoxigenic Escherichia coli F18

产肠毒素大肠杆菌 腹泻 粪便 断奶 生物 戊酸盐 生理学 医学 内科学 微生物学 动物科学 食品科学 大肠杆菌 肠毒素 发酵 丁酸盐 生物化学 基因
作者
Lauren Kovanda,Sofia Rengman,Snehal Tawde,Jeroen Pos,Sangwoo Park,Shuhan Sun,Jungjae Park,Kwangwook Kim,Xunde Li,Yanhong Liu
出处
期刊:Journal of Animal Science [Oxford University Press]
卷期号:102
标识
DOI:10.1093/jas/skae322
摘要

Abstract In the commercial swine farm setting, the postweaning period is a critical window during which piglets are highly susceptible to infection and enterotoxigenic E. coli (ETEC)-associated diarrhea. Short-chain fatty acids and their glycerides are compounds that may influence intestinal health; however, valerate is one that has not been well-characterized for its role as a dietary supplement. Therefore, the major objective of this experiment was to investigate two forms of valerate glycerides on diarrhea, intestinal physiology, and systemic immunity of weaned pigs experimentally infected with ETEC F18. Dietary treatments included a control diet and three additional diets supplemented with 0.075% monovalerin, 0.1% monovalerin, or 0.1% trivalerin, respectively. Piglets were weaned (21 d to 24 d of age), individually housed, and experimental diets were fed through the 28-d trial period. After a 7-d period, all piglets were inoculated on three consecutive days with 1010 CFU ETEC F18/3 mL. Growth performance was monitored throughout the trial, and daily diarrhea scores were recorded. Rectal swabs were collected for bacterial culture to confirm the presence or absence of β-hemolytic coliforms throughout the trial. Serum samples were collected and analyzed for inflammatory biomarkers on days 0, 3, 6, and 21 postinoculation (PI) and untargeted metabolomics on day 6 PI. Intestinal mucosa and tissue sections were harvested from pigs sacrificed on day 7 PI for gene expression and histology analysis. All data, except for frequency of diarrhea and metabolomics, were analyzed by ANOVA using the PROC MIXED of SAS. Dietary trivalerin reduced (P < 0.05) the frequency of severe diarrhea over the entire trial period and the frequency of β-hemolytic coliforms on day 7 PI compared with the control. The intestinal villus height on day 7 PI in jejunum tissue was increased (P < 0.05) in pigs fed trivalerin. The mRNA expression of TNF-α was decreased (P < 0.05) in the trivalerin group, while that of ZO1 was increased (P < 0.05) compared with control. Throughout the trial, serum TNF-α was reduced in pigs fed trivalerin compared with control. Serum metabolites, adenosine, inosine, and shikimic acid were reduced (P < 0.05) on day 6 PI in all treatment groups compared with control. In conclusion, the present results indicate supplementing dietary valerate glycerides exhibited beneficial impacts on diarrhea, inflammation, and intestinal gene expression of piglets during the postweaning period.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
安诺完成签到,获得积分10
刚刚
2秒前
EddieDream发布了新的文献求助10
2秒前
雪1214发布了新的文献求助10
3秒前
3秒前
纳斯达克发布了新的文献求助10
4秒前
5秒前
ying完成签到,获得积分10
5秒前
mingming发布了新的文献求助10
6秒前
Akim应助soldatJiang采纳,获得10
7秒前
田様应助李远哲采纳,获得10
9秒前
10秒前
虾米发布了新的文献求助10
10秒前
人间理想完成签到,获得积分10
12秒前
汉堡包应助缥缈易槐采纳,获得10
12秒前
mingming完成签到,获得积分10
12秒前
隐形的雨安完成签到,获得积分10
13秒前
天天快乐应助Lucky采纳,获得30
13秒前
木光发布了新的文献求助10
14秒前
14秒前
15秒前
15秒前
EXO发布了新的文献求助10
16秒前
16秒前
李远哲完成签到,获得积分10
17秒前
17秒前
17秒前
17秒前
18秒前
可爱的函函应助风清扬采纳,获得10
18秒前
好好发布了新的文献求助10
19秒前
ww完成签到,获得积分10
20秒前
打打应助虾米采纳,获得10
20秒前
贰叁发布了新的文献求助10
21秒前
Inory007发布了新的文献求助10
22秒前
隐形曼青应助未道采纳,获得10
23秒前
小周发布了新的文献求助10
23秒前
ZDY完成签到,获得积分10
24秒前
NL14D发布了新的文献求助10
25秒前
25秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988838
求助须知:如何正确求助?哪些是违规求助? 3531250
关于积分的说明 11252914
捐赠科研通 3269838
什么是DOI,文献DOI怎么找? 1804820
邀请新用户注册赠送积分活动 881943
科研通“疑难数据库(出版商)”最低求助积分说明 809028